The medical progress enables us to transplant many internal organs and tissues. To transplant the whole or some part of an organ, however, the donor sites are limited and the organs to be donated are not always on hand. Therefore, in the field of bone graft, various methods,which as homograft and heterograft, have been tried as well various bone preservation methods like dimineralization, and freeze-dry method. As the surgical skill and technique in cranio-facial field have developed and increased in number recently, the ncessity of the bone graft have not been clarified and clearly understood. Furthermore, there are some clinical problems as followings ;
First, the limitation of the donor sites. Second, bone formation of grafted bone, resorption after the bone graft, and growth potential. Third, the availability of the proper form and size.
It is the aim of this study to find the way of solution of above problems effectively. The osteoconduction process was revealed recently and the successful use of artificial bone substitute as hydroxyapatite clinically and the importance of the fibrin in healing process are reported. By making the desired form with mixture of bone dust and the fibrin sealant without the functional loss on the donor sites, we compared with the classical bone graft in animal experiment.
New Zealan white rabbits were used as experimental animals. We divided the rabbits into 4 groups. The first group consists of the rabbits which have the artificial 8mm defect on the parietal bone as control. The second group consists of the rabbits in which the defect is sealed up by the fibrin sealant only. The third group comprises the rabbits autografted by the template made by mixing the bone dust with the fibrin sealant. The fourth group comprises the rabbits autografted by the bone dust only. The results were carefully examined grossly and microscopically with the periodical biopsies in each group from post-operative third day to third week. The results of the experiment are as followings ;
1. The bony defect was sealed up with the fibrin sealand itself or the template mixed with fibrin sealant and bone dust, mostly the defects were substituted by a new bone around second week after operation.
2. In case of the graft with dust only, the bone dust was not absorbed even by the third week after operation, but more prominent hyperplasia of the osteoblast were neted compared with the group in which the defect was left without it.
3. In the control group, new bone was formed around the peripheral area only on the third week after operation.
In conclusion,
I. A mixture of fibrin sealant and autogenous bone dust plays an effective role to form a new bone in bone healing process.
2. Hopefully the new bone substitutes composing of fibrin sealant and autogenous bone dust, could be used conveniently and easily in many clinical situation.
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